Figure 6.

Expressions analysis of JA synthesis enzyme genes in fertility-reverse plants treated by MEJA/SHAM-spraying treatment. The qPCR analysis of LOX, AOS, AOC and OPR in the spikelets from NK58S and D52S sterile/fertile/fertility-reverse lines during 5th–7th-stage. The internal reference gene is OsActin. By photoperiod conditions and spraying treatment, the LD/SD-treated plants are marked with blue/yellow background, respectively. Data are shown as mean ± standard deviation (n = 3). Asterisks indicate significant differences of gene expression between spraying-induced reversed-fertility plants and those in the control group at the same period revealed by Student’s t-test (*, p < 0.05; **, p < 0.01). LD, long-day cycle; SD, short-day cycle; MEJA, methyl jasmonate; SHAM, salicylhydroxamic acid; CK, control; S/F/R, sterile/fertile/fertility-reverse plants; 5/6/7, 5th/6th/7th-stage.