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. 2021 Jul 31;26(15):4658. doi: 10.3390/molecules26154658

Figure 1.

Figure 1

Nanoconjugate synthesis and characterization. (A) Synthetic route to produce antibody-MORF1, Fab’-MORF1 and HSA-(MORF2)y conjugates. Note: If not otherwise stated all conjugates contain a diethyleneglycol unit in the spacer inserted by SM-PEG2. Conjugates containing longer spacers are denoted as, e.g., Fab’-PEG8-MORF1. (B) Nanoconjugate size exclusion chromatography profiles detected on a Superdex 200 10/300 GL column, PBS (pH 7.4) as eluant at 0.4 mL/min flow rate. (C) Characterization (MORF valence) of nanoconjugates determined using UV-Vis absorbance at λ = 260 nm and BCA assay.