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. 2021 Jul 29;22(15):8130. doi: 10.3390/ijms22158130

Figure 3.

Figure 3

Real-time spreading of ARPE-19 cells under ATP synthase inhibition. (A) Normalized capacitance across ARPE-19 cells vs. time, measured at an AC current frequency of 64,000 Hz. Treatments were applied at time T = 0. ARPE-19 cell groups were control, 1 µM, and 10 µM oligomycin treatment. Capacitance was measured from time of placement onto electrode to 100 h after treatment application, and capacitance values were normalized to that at T = 0. (B) Bar chart representation of the normalized capacitance of each of the cell groups at time T = 100 h, the experiment’s endpoint. (C) Bar chart representation of the area under the normalized capacitance curve of the ARPE-19 cell groups for the interval T = 0–100 h. Compared to the control, the oligomycin 10 µM group affects cell spreading behavior on average across treatment duration, but no effect remains by the end of the experiment. Abbreviations: AUC, area under the curve; Norm, normalized; ns, no significance. Data shown are the mean ± SD of 5–6 independent biological replicates per group. p values are **** p ≤ 0.0001.