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. 2021 Jul 28;22(15):8057. doi: 10.3390/ijms22158057

Figure 3.

Figure 3

Effects of ECE and DK on pyroptosis by reducing NLRP3 inflammasome formation in the gastrocnemius muscle of the Dexa-induced muscle atrophy model. (a,b) Protein levels of NLRP3 inflammasome-pyroptosis-related molecules (NLRP3, ASC, Cas1, c-Cas1, IL-1β, GSDMD, and cleaved GSDMD) were determined by Western blotting. As a result, NLRP3 inflammasome-pyroptosis-related molecules were increased by Dexa but decreased by ECE and DK. (c,d) mRNA levels of Murf1 and Atrogin-1, related to muscle protein degradation, were increased by Dexa but decreased by ECE and DK. The dose unit of ECE and DK is mg/kg/day. a–f: Different letters indicate significant differences among groups, as determined by multiple comparison (Mann–Whitney U test); p < 0.05. ASC, adaptor molecule apoptosis-associated speck-like protein containing CARD; Cas1, caspase 1; c-Cas1, cleaved-caspase 1; c-GSDMD, cleaved-gasdermin D; Dexa, dexamethasone; DK, dieckol; ECE, Ecklonia cava extract; GSDMD, gasdermin D; IL-1β, interleukin-1beta; NLRP3, NOD-like receptor pyrin domain-containning protein 3.