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. 2021 Jul 28;22(15):8107. doi: 10.3390/ijms22158107

Figure 2.

Figure 2

p38 signaling regulates the effect of visfatin on GSK3β inactivation and subsequent COX2 expression in human primary chondrocytes. (A) Human primary chondrocytes were kept as controls (CL) or treated with 5 μg/mL of visfatin for 1, 4, 8 and 24 h, and then the p38-Thr180/Tyr182 and ERK1/2-Thr202/Tyr204 kinases phosphorylations were analyzed by Western blot. (B,C) Human primary chondrocytes were pretreated with DMSO, PD98059 (ERK1/2 inhibitor, 30 μM), or SB203580 (p38 inhibitor, 10 μM) for 1 h and then kept as controls (CL) or treated with 5 μg/mL of visfatin (Vis) for 8 h. COX2 expression (B) and GSK3β-Ser-9 phosphorylation (C) in human primary chondrocytes were analyzed by Western blot. Results in (A–C) are representative of three independent experiments with similar results. Statistic data in (A–C) are mean ± SEM from three independent experiments. *, p < 0.05 vs. untreated control cells (CL) or DMSO-CL-treated cells. #, p < 0.05 vs. DMSO-visfatin (Vis)-stimulated cells.