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RANKL-induced osteoclastogenesis of RAW264.7 cells for 3 days. Experimental design of osteoclastogenesis using porous scaffold (right above). (A) optical images of TRAP+ cells (scale bar = 100 μm). (B) quantification of TRAP activity. The differences were considered significant when ns = not significant (p ≥ 0.05), *** p < 0.001, and # p < 0.0001 (n ≥ 3).
