Figure 1.

Stromal vascular fraction (SVF) cell-based constructs cultured in perfusion or static condition. (A) Immunostaining for endothelial cell marker PECAM-1 (CD31, green), proliferating cell marker Ki67 (red) of SVF cells cultured either in static or perfusion condition at either 21% or <1% O₂. Cell nuclei were stained with DAPI (blue). Scale bar = 75 µm. (B) Percentage of proliferating cells for static and perfusion conditions cultured under in vitro normoxia and hypoxia. n = 12 (1 donor). **** p < 0.0001. * p = 0.049.