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. 2021 Jul 24;22(15):7920. doi: 10.3390/ijms22157920

Figure 4.

Figure 4

In vitro characterization of stromal vascular fraction (SVF)-based constructs. SVF composition and angiogenic factor release. (A) Flow cytometry analysis: percentage of different SVF subpopulations, namely, pericytic-like (CD45−, CD34−, CD146+), mesenchymal stromal cells (MSC, CD45−, CD90+, CD73+), and endothelial progenitor (CD45−, CD31+, CD34+) and mature (CD45−, CD31+, VEGFR2+) cells, following 3D perfusion-based culture in in vitro normoxia or hypoxia. Values are presented as percentage of living cells. n.s. = not statistically significant. n = 5. (B) Quantification of human VEGF released in the supernatant by SVF cultured under severe hypoxia or normoxia culture normalized by the total DNA content. ** p < 0.001. n = 4. hVEGF: human vascular endothelial growth factor; DNA: desoxyribonucleic acid. Comparison was performed with non-parametric test (Mann–Whitney-U).