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. 2021 Aug 2;22(15):8313. doi: 10.3390/ijms22158313

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Expression of NUCB2/NESF-1 in invasive ductal breast carcinoma (A) and mastopathy (B) NUCB2/NESF-1 was located in the cytoplasm of breast cancer cells (black arrows) (A). Original magnification: ×200; for the immunohistochemical demonstration of NUCB2/NESF-1, Autostainer Link 48 (Dako, Glostrup, Denmark) with the visualization system of EnVisione FLEX, High pH (Dako) was used. NUCB2 was detected by incubation of deparaffinized sections with primary anti-nucleobindin 2 antibodies (rabbit polyclonal antibody, Novus Biologicals, Littleton, CO, USA).