Figure 4.

Effects of amber extract treatment on adipocytokine-associated gene expression in 3T3-L1 cells. Mature 3T3-L1 cells treated with amber extract (0, 10, 25, and 50 µg/mL) were collected after 96 h, in which mRNA levels were measured by RT-PCR, and protein levels were detected by western blotting. Expression of (A) adiponectin and (C) leptin at the mRNA level was detected by RT-PCR; the data were directly obtained from the machine. Western blot images of (B) adiponectin analyzed by ImageJ. Statistical analysis of western blot bar graphs was performed in three independent experiments. The results are presented as the mean ± SD; n = 3; * p < 0.05, *** p < 0.001 vs. 0 µg/mL.