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. 2021 Jul 30;22(15):8206. doi: 10.3390/ijms22158206

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Analysis of the Focus Expansion Assay (FEA) on epithelial cells (ARPE) (A–C) and fibroblasts (HFFs) (D–F) with plasma obtained from D−/R+ (A,D), D+/R+ (B,E) and D+/R− (C,F) lung transplant recipients (LTRs) after lung transplantation (LTX). D−/R+ and D+/R+ LTRs were tested at 0, 3, 6, 9 and 12 months post LTX. D+/R− LTRs were tested at 0, 3, 6, 9, 12, 15, 18, 21 and 24 months post LTX. Data are shown as mean values (± SEM). The mean value of the HCMV-seropositive control cohort (N = 80) is indicated as black triangle (± SEM). Plaque sizes at each time point were compared between LTRs and seropositive control patients by ANOVA and Dunn’s post-test; p < 0.05 was considered significant. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. IE⁺: HCMV-immediate early positive cells.