Figure 2.

Representative images of PCR genotyping (a,b) and Connexin (Cx43)-del PCR (c). (a) Identification of loxP site expression showing no loxP site (=wild type [WT]) and both alleles flanked by loxP sites (“homoflox”); (b) Identification of Cre expression with Cre- negative mice showing no band and Cre-positive mice showing a single band at approximately 470 base pairs (bp) (given example was from premeiotic germ cell specific Cx43 knockout (KO) (pGCCx43KO) mice); (c) Confirmation of the deletion of Cx43 in testis homogenate was performed by Cx43-del PCR. Primers generated a 670 bp amplicon in KO but not in WT mice (given example was from pGCCx43KO mice).