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Antibodies used for immunohistochemistry (IHC) and Western blot (WB) analysis.
| Antibody | Host | Application | Dilution | Company | Catalogue No. |
|---|---|---|---|---|---|
| Anti-Sox9 | Rabbit | IHC | 1:800 | Merck Millipore | AB5535 |
| Cx43 | Rabbit | IHC IF |
1:500 | Cell Signaling | 3512 |
| WB | 1:1000 | ||||
| α-Tubulin | Rabbit | WB | 1:1000 | Cell Signaling | 2125 |
| 2nd Anti-Rabbit | Goat | WB | 1:5000 | Santa Cruz | SC-2004 |
| EnVision+ Single Reagent (HRP Rabbit) | Goat | IHC | ready-to-use | Agilent Dako | K400311-2 |
| 2nd Alexa 546 | IF | 1:1000 | Invitrogen | A11010 |
