Table 1.
Rearing efficiency and female lethality of L. cuprina TSS
| TSS | Condition | Teta | #Pupae | #Male | #Female | #Adult | AERb | %Male |
|---|---|---|---|---|---|---|---|---|
| FL3#2c | Heterozygous | – | 2098 | 644 | 735 | 1379 | 65.7 | 46.7% |
| + | 2468 | 1068 | 1048 | 2116 | 85.1 | 50.5% | ||
| Homozygous | – | 2387 | 1029 | 1 | 1030 | 43.1 | 99.9% | |
| + | 1647 | 672 | 701 | 1373 | 83.3 | 49.0% | ||
| FL3#2d | Homozygous | – | 1736 | 881 | 2 | 883 | 50.9 | 99.8% |
| + | 1562 | 629 | 685 | 1314 | 84.1 | 47.9% | ||
| DH6d(FL3#2; EF1#12) | Heterozygous | – | 1717 | 1383 | 0 | 1383 | 80.5 | 100% |
| Homozygous | – | 427 | 270 | 0 | 270 | 63.2 | 100% | |
| ++/− | 1421 | 885 | 0 | 885 | 62.3 | 100% | ||
| + | 1611 | 719 | 669 | 1388 | 86.2 | 51.8% | ||
| DH1e(DR2#6; EF1#12) | Heterozygous | – | 1091 | 916 | 0 | 916 | 84 | 100% |
| Homozygous | +/− | 1054 | 958 | 0 | 958 | 90 | 100% | |
| + | 2719 | 1284 | 1234 | 2518 | 92.6 | 51.0% | ||
| DH2e(DR2#6; EF3E) | Heterozygous | – | 1252 | 1099 | 0 | 1099 | 87.8 | 100% |
| Homozygous | +/− | 694 | 594 | 0 | 594 | 85.6 | 100% | |
| + | 1724 | 784 | 738 | 1522 | 88.3 | 51.5% | ||
| DH3f(DR3#2; EF3E) | Heterozygous | – | 1082 | 771 | 0 | 771 | 71.3 | 100% |
| Homozygous | +/− | 1158 | 981 | 0 | 981 | 84.7 | 100% | |
| + | 1949 | 838 | 810 | 1648 | 84.6 | 50.8% | ||
| DH4f(DR3#4; EF1#12) | Heterozygous | – | 2398 | 1690 | 30 | 1720 | 71.7 | 98.3% |
| Homozygous | +/− | 953 | 801 | 0 | 801 | 84.1 | 100% | |
| + | 2284 | 1039 | 1008 | 2047 | 89.6 | 50.8% | ||
| DH5f(DR5#4; EF1#12) | Heterozygous | – | 1271 | 1132 | 0 | 1132 | 89.1 | 100% |
| Homozygous | – | 660 | 561 | 0 | 561 | 85 | 100% | |
| + | 2162 | 981 | 881 | 1862 | 86.1 | 52.7% |
a “-” stands for no tetracycline in the diet, and “+” stands for plus tetracycline in the diet, “+/−” indicates parents fed a low dose of tetracycline (1 or 3 μg/mL for the first 2 days), “++/−” indicates a high dose of tetracycline (100 μg/mL) was supplied to the parental adults for the first 8 days but not their progeny that were counted
bAER stands for adult emergence ratio
cData from [20]. Eggs were collected up to two times from 10 to 20 pairs of adults
dData from this study, three replicates of 8-pairs per cage
eData from [12]. For DR2 the bottleneck (bnk) cellularization gene promoter from L. sericata was used to drive expression of tTA. EF3 contains the wild type version of Lshid whereas EF1 has a phosphomutated version called LshidAla2
fData from [31]. The spitting image (spt) gene promoter from L. sericata and the actin5C gene promoter from L. cuprina was used to drive expression of tTA for DR3 and DR5, respectively