Figure 2.

PD-1 expression on CD3⁺ and CD8⁺ T cells in ICI responders and non-responders. PBMC were taken from patients at indicated time points as described in Figure 1 and analyzed by flow cytometry (FACS). (A,D) PD1⁺CD3⁺ or PD1⁺CD8⁺ expression without stimulation. (B,E) PD1⁺CD3⁺ or PD1⁺CD8⁺ expression in control cells after cell culture in 10% FCS. (C,F) PD1⁺CD3⁺ or PD1⁺CD8⁺ expression after anti-CD3/anti-CD28 stimulation. Nominal (unadjusted) p-values (Mann–Whitney U test between responders and non-responders) are shown without adjustment for multiple testing. Responders are indicated by response = 1, non-responders by response = 0. Data in (A–F) are given as percentage of PD-1⁺ cells of total CD4⁺ and CD8⁺ T cells, respectively.