Figure 4.

Inflammatory T cell subpopulations in patients with and without irAE. PBMC were taken from patients at indicated time points as described in Figure 1 and analyzed by flow cytometry (FACS). (A,B) Percentage of CD4⁺ and CD8⁺ effector T cells. (C) CD4⁺CD38⁺HLADR⁺ T cells. (D) CD8⁺CD38⁺ T cells. irAE, immune-related adverse events. 0, without; 1, with adverse events. Nominal (unadjusted) p-values (Mann–Whitney U test between irAE = 1 and irAE = 0) are shown without adjustment for multiple testing. Data in (A,B) are given as percentage of CD4⁺ effector cells and CD8⁺ effector cells, respectively, of total CD4⁺ and CD8⁺ cells. Data in (C) are given as percentage of CD38⁺HLADR⁺ cells of total CD4⁺ cells. Data in (D) are given as percentage of CD38⁺ cells of total CD8⁺ cells.