| FC | flow cytometer |
| FI | fluorescence intensity, height of a fluorescence pulse, volts or digital unit |
| MFI | mean fluorescence intensity |
| SD | standard deviation of the fluorescence signals |
| CV | coefficient of variation, SD divided by MFI. |
| CV% | the product of CV and 100 |
| PMT | photomultiplier tube |
| CVC | current-to-voltage converter circuit |
| ADC | analog-to-digital converter circuit |
| Gri | ground-state occupation index of the i-th label (1 if occupied, 0 if not occupied) |
| X, Y, Z | names of the axis used to describe the geometry of the flow, laser propagation, and detector |
| x, y, z | specific values of X-, Y-, Z-coordinates |
| L, M, H | names of three beads, with low, medium, and high label loadings |
| PBMCs | peripheral blood mononuclear cells |
| mAbs | monoclonal antibodies |
| FITC | fluorescein isothiocyanate, derivative of fluorescein, a fluorescent molecule |
| R-PE | R-phycoerythrin, fluorescent protein isolated from red algae |
| CD19 | receptor found on the surface of B cells |