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. 2021 Jul 27;22(15):7992. doi: 10.3390/ijms22157992

Figure 2.

Figure 2

(A) Design of the PAM-out strategy to target human MIR146B with two sgRNAs in opposite strands at optimum distance. (B) Human MIR146B locus highlighting the precursor miR-146b sequence, and the mature strands miR-146b-5p and 3p are underlined. SgRNA A and B target sequences are shown in blue and the PAM sequences in red. Single-strand DNA break point due to Cas9n (D10A) nickase activity is indicated by red triangle. Figure (A) was modified from idtdna.com to include miR-146b sgRNA design.