Fig. 1.

Neuronal Dystroglycan is not required for pyramidal neuron migration. (a) Schematic of Dystroglycan on pyramidal neurons. Inset shows the structure of Dystroglycan and sugar chain moieties present on the extracellular subunit. (b) Mouse breeding scheme for generating pyramidal neuron-specific Dag1 conditional knockout mice using Nex^Cre driver mice. (c) Immunostaining for Dystroglycan in the hippocampal CA1 region of P30 Dag1^Control mice (left panel) shows punctate Dystroglycan protein on the soma and proximal dendrites of pyramidal neurons, whereas Dag1^cKO mice (right panel) lack perisomatic staining. Asterisks denote Dystroglycan staining on blood vessels which is retained in Dag1^cKO mice. (d) Coronal sections from P15 Dag1^Control and Dag1^cKO cortex were immunostained for upper layer marker CUX1 (L2–4). (e) Coronal sections of the cortex from P30 Dag1^Control and Dag1^cKO mice crossed with a Thy1^YFP reporter mouse to sparsely label layer 5–6 pyramidal neurons (green) and stained for Calbindin (magenta) to label layer 2–3 pyramidal neurons. (f) Coronal sections of the hippocampus from P30 Dag1^Control and Dag1^cKO mice crossed with a Thy1^YFP reporter mouse to label excitatory neurons (green) in the CA regions and dentate gyrus