Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 6;16:4. doi: 10.1186/s13064-021-00153-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — CCK+ interneurons are selectively reduced in mice lacking Dystroglycan from pyramidal neurons. (a-b) Immunostaining for medial ganglionic eminence (MGE)-derived interneuron markers (green) parvalbumin (PV) (a) and somatostatin (SOM) (b) show normal innervation of the hippocampus in P30 Dag1^Control and Dag1^cKO mice. Insets (yellow boxed regions) show enlarged images of the CA1. (c-d) Immunostaining for caudal ganglionic eminence (CGE)-derived interneuron markers (green) Calretinin (c), and CB₁R (d) show normal innervation of Calretinin interneurons in Dag1^Control and Dag1^cKO mice, whereas CB₁R is largely absent from the CA regions of Dag1^cKO mice. Insets (yellow boxed regions) show enlarged images of the CA1. (e) Immunostaining for CB₁R in hippocampal CA1 (top) and CA3 (bottom) of P30 Dag1^Control and Dag1^cKO mice. (f) Quantification of CB₁R pixels for each CA layer of the CA1 and CA3 shows a significant reduction in CB₁R staining in Dag1^cKO mice (*P < 0.05, unpaired two-tailed Student’s t-test; n = 4 mice/genotype). Data are presented as mean values ± s.e.m. Data are normalized to Dag1^Control signal in each CA layer. CA layers: SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum