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. 2021 Aug 6;16:4. doi: 10.1186/s13064-021-00153-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Post-developmental maintenance of CCK+ interneurons does not require Dystroglycan. (a) Breeding scheme and experimental approach for generating tamoxifen-inducible Dystroglycan conditional knockout mice. Dag1^Ctrl;Camk2a^CreERT2;Ai9 and Dag1^icKO;Camk2a^CreERT2;Ai9 mice were treated with tamoxifen (5 mg/ml) at P23 and brains were collected for immunohistochemistry 6 weeks later at P65. (b) Single channel images of tdTomato staining in the hippocampus show the recombination pattern in PyNs. Insets show enlarged view of tdT+ pyramidal neurons in the CA1. (c) Immunostaining for CB₁R+ terminals (green) and tdTomato signal (magenta) in the hippocampus of P65 Dag1^Ctrl;Camk2a^CreERT2;Ai9 (left panels) and Dag1^icKO;Camk2a^CreERT2;Ai9 mice (right panels) shows that the deletion of Dystroglycan in adult PyNs does not affect CB₁R+ terminal maintenance. (d) Quantification of CB₁R pixels in hippocampal CA1 of Dag1^Ctrl;Camk2a^CreERT2;Ai9 (gray) and Dag1^icKO;Camk2a^CreERT2;Ai9 (pink) mice (n.s. = not significant, unpaired two-tailed Student’s t-test; n = 3 mice/genotype). Data are presented as mean values ± s.e.m. Data are normalized to Dag1^Control signal in each layer. SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum