Table 3.
Impact of alleviation treatments on growth parameters of chilling-stressed coriander plants at the flowering stage. Coriander control plants and chilling-stressed ones were subjected to pot. Silicate (80 mM), HA (50 mgl−1) or γ-radiation (50 Gy). The shown data was extracted by using 3 biological and 3 technical replicates. Each biological replicate is comprised of 10 plants (one pot). The mean value of each biological replicate represents the value of one technical replicate. The readings of the 3 technical replicates were recorded. The mean value of the technical replicates was used to calculate ±SE. Also, the least significant differences (LSD) at 5% level were calculated to compare the means of different treatments according to Snedecor and Cochran [92]. The values with the same letter are not significantly different (P<0.05). The raw data set of the technical replicates was attached as a supplementary file. By being the best alleviation element against the chilling stress, the percentage of increase (inc.) in all measurements, triggered by HA application, was further investigated. This percentage was calculated by subtracting the value of control/chillied reading from the reading value of any physiological treatment, then the result was divided by the reading of control value, and finally, the result is multiplied by 100
| Growth Parameters | Shoot length (cm) | No. of leaves/ plant | No. of branches /plant | Leaves area/plant (cm)2 | F. wt. of root (g) | D. wt. of shoot (g) | Root length (cm) | F. wt. of shoot | D. wt. of root (g) | No. of inflorescence/plant |
|---|---|---|---|---|---|---|---|---|---|---|
| Treatment | ||||||||||
| Control | 32 ± 1.5d | 69.67 ± 1.45d | 7.33 ± 0.33d | 39.31 ± 0.43e | 2.62 ± 0.1d | 0.480 ± 0.025ed | 9.17 ± 0.4de | 0.136 ± 0.008e | 0.082 ± 0.006f | 7 ± 0,57cd |
| Chilling (6 °C ± 0.5) | 27.9 ± 0.49e | 40.33 ± 1,45e | 5 ± 0.57e | 14.28 ± 0.71f | 2.14 ± 0.24d | 0.314 ± 0.005 e | 9.73 ± 0.2 cde | 0.137 ± 0.01e | 0.0495 ± 0.002g | 5 ± 0.57d |
| Pot. silicate (80 mM) | 36.67 ± 1.2 c | 103.33 ± 2.4a | 9 ± 0.57bcd | 94.29 ± 0.82b | 4.83 ± 0.088b | 0.926 ± 0.04b | 12.07 ± 1ab | 0.319 ± −.04c | 0.114 ± 0.01d | 11 ± 0.57ab |
| Humic acid (50 mg.l−1) | 46 ± 1.5a | 101 ± 0.58a | 12 ± 0.57a | 108.84 ± 3.17a | 7.2 ± 0.65a | 1.686 ± 0.16a | 13.37 ± 0.4a | 0.93 ± 0.088 a | 0.245 ± 0.007a | 12 ± 0.57a |
| γ-ray (50 Gy) | 40.17 ± 0.44b | 89.67 ± 1,45b | 9.67 ± 0.88bc | 90.31 ± 1.5b | 3.87 ± 0.32bc | 0.8129 ± 0.02bc | 11.23 ± 0.8bc | 0.474 ± 0.038b | 0.197 ± 0.01b | 8.67 ± 0.88c |
| Chilling + Pot. silicate | 34 ± 0.57cd | 80.33 ± 4.6c | 8 ± 0.57cd | 57.39 ± 1.14d | 2.41 ± 0.06d | 0.473 ± 0.06 de | 10.2 ± 0.15cd | 0.194 ± 0.006de | 0.0823 ± 0.003f | 6.33 ± 0.88d |
| Chilling + Humic acid | 36 ± 1.52c | 93.67 ± 1.8b | 10 ± 0.57b | 71.82 ± 1.5c | 4.1 ± 0.25b | 0.979 ± 0.04b | 10.6 ± 0.3bcd | 0.433 ± 0.02 b | 0.138 ± 0.02c | 9 ± 0.57bc |
| Chilling + γ-ray (50 Gy) | 28.33 ± 0.88e | 80 ± 1.15c | 8 ± 0.29cd | 59.34 ± 3.2d | 3.07 ± 0.37 cd | 0.608 ± 0.03cd | 9.57 ± 0.57d | 0.285 ± 0.008cd | 0.108 ± 0.003e | 8 ± 0.57cd |
| LSD at 0.05 | 3.174 | 6.77 | 1.756 | 5.609 | 0.973 | 0.207 | 1.629 | 0.111 | 0.002 | 2.071 |
| % of inc. by HA over control | 12.5 | 15.6 | 56.5 | 103.9 | 218.4 | 68.3 | 34.4 | 36.4 | 82.7 | 28.6 |
| % of inc. by HA over chilling-stressed | 29.3 | 8.94 | 91.6 | 208.5 | 216.1 | 178.8 | 132.3 | 100 | 402.9 | 80 |