Fig. 8.

Pregnancy exposure to TiO₂NPs resulted in anxiety-like behavior in offspring at PD49 but not presented at PD21. After pregnancy exposure to TiO₂NPs, the anxiety-like behavior in offspring was evaluated by elevated plus maze at postnatal day (PD) 21 and PD49. A Effects of TiO₂NPs on the duration in the open arms. B Effects of TiO₂NPs on the distance in the open arms. C Effects of TiO₂NPs on the frequency of entries into open arms. D The representative track maps in the elevated plus maze. E Effects of TiO₂NPs on the head dips. Data were presented as mean ± S.E.M. Statistical analysis was performed by using repeated-measure ANOVA or independent student t test or Mann–Whitney U test