Fig. 2.

Knockdown of HSPA8 ameliorates neurological dysfunction and histopathological damage of SCII rats. A BBB scoring method was used to assess the SCII rats’ neurological function after 24 h of reperfusion. B The relative protein level of HSPA8 in spinal cord tissues of SCII rats was detected after injection with LV-sh-HSPA8 via western blot assay. C Representative section for HE-stained spinal cord tissues after SCII was assessed. Scale bar = 100 μm. D Representative section for nissl-stained spinal cord tissues after SCII was assessed. Scale bar = 100 μm. E Quantification of HE-staining normal neuronal number after SCII was analyzed. F Quantification of extravascular Evans blue after SCII was analyzed. Data were shown as means ± SD of three independent experiments. N = 6 rats per group. Statistical analysis was performed by one-way ANOVA followed by Turkey’s for multiple comparisons (A, E, and F) or two-tailed unpaired Student’s t-test between two groups (B). *p < 0.05 vs. sham. ^# p < 0.05 vs. SCII + LV-sh-NC. BBB, Basso, Beattie, and Bresnahan; HE, hematoxylin–eosin; SCII, spinal cord ischemia–reperfusion injury