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. 2021 Aug 6;18:170. doi: 10.1186/s12974-021-02220-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Knockdown of HSPA8 inhibits NLRP3 inflammasome activation and phosphorylation of NF-κB in SCII rats. A Immunofluorescence of GFAP in spinal cord tissues after SCII was performed. Scale bar = 50 μm. B Quantification of GFAP-positive cells after SCII was analyzed. C Relative protein levels of NLRP3, ASC, and p20/pro-caspase-1 in spinal cord tissues of SCII rats were detected after injection of LV-sh-HSPA8 via western blot assay. D The contents of IL-1β and IL-18 in the spinal cord tissue homogenate were detected by ELISA assay. E Relative protein levels of total NF-κB P65 and p-NF-κB P65 in spinal cord tissues of SCII rats were detected after injection LV-sh-HSPA8 via western blot assay. F The intracellular location of the NF-κB P65 in spinal cord tissues of SCII rats was observed by immunofluorescence staining. Scale bar = 50 μm. G The DNA-binding activity of NF-κB in spinal cord tissues of SCII rats was revealed by EMSA. Data were shown as means ± SD of three independent experiments. N = 6 rats per group. Statistical analysis was performed by one-way ANOVA followed by Turkey’s for multiple comparisons. *p < 0.05 vs. sham. ^# p < 0.05 vs. SCII + LV-sh-NC. NLRP3, nod-like receptor pyrin domain-containing 3; ASC, the apoptosis-associated speck-like protein containing CARD; SCII, spinal cord ischemia–reperfusion injury; IL-1β/18, interleukin-1β/18; NF-κB, nuclear factor-kappa B