Materials
| Name | Company | Catalog Number | Comments |
|---|---|---|---|
| 2 mL glass LC vial | Microsolv | 9502S-WCV | Vessel to hold PCR tube for autosample injection |
| BSA | Sigma-Aldrich | P0834-10×1mL | Carrier protein for greatly reducing surface adsorption losses |
| DTT | Thermo Scientific | A39255 | Reagent for reduction |
| Formic acid | Thermo Scientific | 28905 | Reagent for stopping enzyme reaction |
| IAA | Thermo Scientific | A39271 | Reagent for alkylation |
| Peptide internal standards | New England peptide | Targeted quantification of EGFR/ MAPK pathway proteins | |
| RT-PCR tube | GeneMate Bioexpress | T-3035-1 | 0.2 mL PCR tube for one-pot sample preparation |
| Skyline software | University of Washington | Publicly available for SRM data analysis | |
| Sonicator | Hielscher Ultrasound Technology | UTR200 | Sonication on ice for cell lysis |
| Speed Vac concentrator | Thermo Scientific | Reduction of the percentage of TFE for effective trypsin digestion | |
| TFE | Sigma-Aldrich | 18370-10×1mL | 60% TFE for cell lysis |
| Thermocycler w/ heated lid | Peltier Thermal Cycler | PTC-200 | Heating for protein denaturation |
| Trypsin Gold | Promega | V528A | Enzyme for protein digestion |
| Waters BEH C18 column | Waters | C18 column for peptide separation |