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. Author manuscript; available in PMC: 2021 Aug 15.
Published in final edited form as: Int J Biol Macromol. 2020 Apr 26;157:158–169. doi: 10.1016/j.ijbiomac.2020.04.199

Fig. 2. Schematic representation of three genetic cassettes created for transformation.

Fig. 2.

Cassette A: Five mammalian genes were cloned into pBI121 expression vector. Resultant cassette contains nos-P::CST::nos-T, 35S-P::ST::nos-T, GapC-P::NANS::GapC-T, 35S-P::GNE::nos-T, and Ubi.U4-P::CMAS::GapC-T with nptII as a selective marker. Cassette B1: Human EPO and chimeric GalT genes were cloned into modified expression vector MpBI121. Resultant cassette contains 35S2-P::EPO::nos-T and nos-P::ST/GalT::nos-T with bar as a selection gene. B2: MGAT3, wild-type GalT and human EPO genes were cloned into modified expression vector MpBI121. Resultant cassette B3 contains 35S2P::EPO::nos-T, GapC-P::MGAT3::GapC-T and nos-P::GalT::nos-T with bar as a selection gene.