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. Author manuscript; available in PMC: 2021 Aug 15.
Published in final edited form as: Int J Biol Macromol. 2020 Apr 26;157:158–169. doi: 10.1016/j.ijbiomac.2020.04.199

Fig. 5. PCR analysis to determine the presence of transgenes including two selection genes in six transgenic lines from each genetic cassette combination.

Fig. 5.

A) Six transgenic lines from genetic cassettes A and B1 were used for confirming the presence of nptII, bar, EPO, ST/GalT, CST, CMAS, ST, NANS, GNE. B) six transgenic lines from genetic cassettes A and B2 used for confirming the presence of nptII, bar, EPO, GalT, CST, CMAS, ST, NANS, GNE and MGAT3. Genomic DNA obtained from leaves of each transgenic line was used for PCR amplification with gene specific primers. For ST/GalT, only GalT part was amplified. P: plasmid DNA containing respective genes.