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. Author manuscript; available in PMC: 2021 Aug 15.
Published in final edited form as: Int J Biol Macromol. 2020 Apr 26;157:158–169. doi: 10.1016/j.ijbiomac.2020.04.199

Fig. 6. RT-PCR to detect transcripts of all transgenes including two selection genes in six transgenic lines from each genetic cassette combination.

Fig. 6.

A) Six transgenic lines from genetic cassettes A+B1 were used to detect transcripts of nptII, bar, EPO, ST/GalT, CST, CMAS, ST, NANS and GNE. B) Six transgenic lines from genetic cassettes A+B2 were used to detect transcripts of nptII, bar, EPO, GalT, CST, CMAS, ST, NANS, GNE and MGAT3. cDNAs made from total RNAs isolated from leaf tissue of transgenic lines were used for RT-PCR amplification. The standard primers from the QuantumRNATM 18S internal standard kit was used to target the 18S rRNA gene to ensure equal loading. For ST/GalT, only GalT part was detected.