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. 2021 Jun 18;3(1):vdab076. doi: 10.1093/noajnl/vdab076

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© The Author(s) 2021. Published by Oxford University Press, the Society for Neuro-Oncology and the European Association of Neuro-Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — LIP internalization and cytotoxicity in patient-derived GSC lines. Established GSC lines were investigated for stemness: (A) Real-time PCR detection of NESTIN and SOX2 genes in GSC lines and human astrocyte cell line (SVGp12) included as controls. Expression data were normalized on GAPDH. Results are shown as mean values ± SEM of triplicates; (B) Representative immunofluorescence of stem markers Nestin (green) and SOX2 (red) in GSC1 xenografts at day 80 from intracranial injection. Nuclei are counterstained with DAPI. Scale bar: 20 μm. (C) Representative images of GCS3 cells incubated with mApoE-DOXO-LIP or DOXO-LIP. (D) Quantification of nuclear DOXO as mean fluorescence intensity (MFI) ± SE normalized to cells incubated with DOXO-LIPs (4 h, DOXO 4 mg/ml). (E) GSC viability after 48 h incubation with LIPs at increasing DOXO concentrations. Values are expressed as mean percentage survival (6 replicates ± SE) normalized to corresponding untreated. *P < .05, ****P < .0001.