Table 2.

Sequences, target size, and melting temperature of primers used

Organisms or group	Target gene	Primer	Primer sequences (5′–3′)	T m 1, °C
Sulfonamide (Sul) resistance	Sul1	Sul1-FW319	5′-CGA TCA GAT GCA CCG TGT T-3′	60
		Sul1-RV430	5′-CGC AGG GTC AGG AAA TCC-3′
Tetracycline (Tet) resistance	TetA	TetA-FW767	5′-CAA CTT GTC GGA CAG GTG -3′	60
		TetA-RV886	5′-GGC GAG TGA ATG CAG AAT-3′

¹T _m melting temperature.