Fig. 4.

Glucose dose-response on I_K1 and effect of changing extracellular K⁺ concentration. a Representative I_K1 traces measured using a ramp protocol (shown above) in the presence of increasing concentrations of glucose in rabbit ventricular myocytes. b Glucose dose–response curves on I_K1 at – 60 mV in control and following pretreatments with either insulin (5 nmol/L) or Thiamet-G (Thm-G, 100 nmol/L). c Representative I_K1 traces in hyperkalemia and hypokalemia. d Hyperkalemia and hypokalemia shifted the reversal potential of I_K1 (E_K) and the voltage, where outward I_K1 peaked. e Representative I_K1 traces in combined hyperkalemia and hyperglycemia. f Hyperglycemia markedly increased I_K1 in hyperkalemia and slightly increased in hypokalemia. Two-way ANOVA; *p < 0.05, **p < 0.01, ***p < 0.001 vs. normoglycemia; ^†††p < 0.001 vs. control