Figure 5.

The Pin1 inhibitor, Juglone, regulated oxidative stress caused by H/R injury in vitro. The model was established with 12 h hypoxia and 6 h reoxygenation. HK-2 cells were treated with the Pin1 inhibitor (0.1,1, and 10 μM) for 1 h and then experienced the H/R process. (a) The effect of different concentrations of juglone on the control group. (b–f) Western blot was used to detect the expression of Pin1, 4-HNE, COX2, and MPO and quantification was performed. (g–j) The SOD, MDA, ROS, and H₂O₂ levels were detected after the H/R process in vitro (n = 5). The values were presented as mean ± SEM. ^∗P < 0.05 vs. the control group and ^#P < 0.05 vs. the H/R group.