Figure 6.

Pin1 silence decreased oxidative stress induced by H/R in vitro. The H/R model was established with 12 h hypoxia and 6 h reoxygenation. The cells were transfected with si-NC or two different si-Pin1 for 24 h and then experienced H/R. (a–e) Western blot was used to detect the expression of Pin1, 4-HNE, COX2, and MPO and quantification was performed. (f–i) The SOD, MDA, ROS, and H₂O₂ levels were detected after H/R in vitro (n = 5). The values were presented as mean ± SEM. ^∗P < 0.05 vs. the control group and ^#P < 0.05 vs. the si-NC group.