Fig. 2.

mRNA (a) and protein (b) expression of alkaline phosphatase (ALP). For RT-PCR and Western blot reactions, Actin was used as inner control. Representative data of 4 independent experiments on days 3, 7, and 21 of callus formation. Optical density (c) of signals was measured and results were normalized to the optical density of WT and PACAP sham-operated tibia. For diagrams a and b, densities and means of four independent experiments (±standard error of the mean) are shown in the figures. Asterisks indicate significant differences compared to sham-operated sides (p < 0.05; one-way ANOVA followed by Tukey’s HSD test)