FIGURE 2.

Induction and maintenance of pluripotency via induced OSKM expression. A, Colony morphology of reprogrammed cells in different inductive media at selected time points. Groups of 2iL and OSKM were shown. Scale bar, 75 μm. B, Colonies observed after reprogramming under inductive media of 2iL and OSKM, respectively. Cells were stained by alkaline phosphatase (AP). C, Morphology of stable cell lines of OSKM‐iPSCs at Passage 23. Scale bar, 75 μm. D, AP staining of OSKM‐iPSCs. Scale bar, 75 μm. E, Karyotype analysis of OSKM‐iPS‐4 cell line. F, Statistical graph of karyological characteristics of the two OSKM‐iPSC lines (OSKM‐iPS‐4 and OSKM‐iPS‐24) at Passage 12. G, Immunostaining for ESC markers OCT4, SOX2, NANOG, and SSEA1 of OSKM‐iPSCs. DNA was stained with Hoechst 33342. Scale bars, 20 μm. H, Histological section analysis of the teratomas derived from OSKM‐iPSCs. Ectoderm, mesoderm, and endoderm structures were shown. Scale bar, 200 μm