FIGURE 3.

Analysis of microgliaand astrocytes in nigrostriatal pathway of mice with partial microglialdepletion after MPTP administration. (A) Immunofluorescencestaining of Iba1⁺ (pink), GFAP⁺ (green) and TH⁺(red) in the SNpc. Scale bars: 200 μm. (B,C) Immunohistochemical staining showing Iba1⁺ (B) and GFAP⁺ (C) cells in the striatum.Scale bars: 100 μm. (D, E) Quantification of Iba1⁺ microglialcells (D) and GFAP⁺ astrocyticcells (E) in the SNpc. n=3‐6. (F, G) Quantification of Iba1⁺ microglial cells (F) and GFAP⁺ astrocytic cells (G) in the striatum. n=7. NS: normal saline; CD: control diet;PLX3397: PLX3397‐formulated diet. Two‐way ANOVA followed by Holm‐Sidak’smultiple comparisons test was used for statistical analysis. ^** P<.01, ^*** P<.001, ^**** P<.0001, vs respective NS groups. ^# P<0.05, ^## P<.01, ^### P<.001, ^#### P<.0001, CD groups vs PLX3397 groups