FIGURE 4.

PKM/E10‐GT mutation inhibits cell proliferation and related gene expression. A, E10‐GT mutation inhibits cell growth. CCK‐8 kit was used to analyse the cell growth of single‐clone‐derived E10‐GT‐mutant cells and wild‐type HCT116 cells served as a control. Absorbance was measured at OD450 at 0, 48 and 96 h. B, RNA‐seq analysis of differentially expressed genes in E10‐GT‐mutant cells relative to wild‐type HCT116 cells. Representative differentially expressed genes were also presented. c‐d. GO C, and KEGG D, pathway analysis of the differentially expressed genes responding to E10‐GT mutation