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. 2021 Jul 7;54(8):e13085. doi: 10.1111/cpr.13085

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© 2021 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Derivation of immunity‐and‐matrix regulatory cells (IMRCs) from human embryonic stem cells (hESCs). A, Different stages of the IMRC derivation protocol. B, Changes in cell morphology during the induction of hESCs into IMRCs. C, The osteogenic, chondrogenic and adipogenic differentiation potential of the IMRC lines generated. D, Flow cytometric analysis of marker expression in IMRCs (passage 5). IMRCs are CD11b−/CD45−/HLA–DR−/CD90+/CD29+/CD73+/CD105+ cells. hEBs, human embryoid bodies. P1, passage 1