FIGURE 2.

Immunity‐and‐matrix regulatory cells (IMRCs) could rescue amyloid‐beta (Aβ)‐induced neural cell damage and lipopolysaccharide (LPS)‐induced inflammation in vitro. (A), IMRCs inhibited LPS‐induced inflammation in a co‐culture model. Schematic of a BV2 and IMRCs co‐culture model and the quantification of mRNA expression. n = 9 repeats, means ±SEM, one‐way ANOVA with Tukey's multiple comparison test; n.s., nonsignificant, **p <.01, ****P <.0001. (B), Conditional medium (CM) of IMRCs reduces LPS‐induced inflammation in BV2 microglial cells. Schematic of the experimental workflow (upper) and the quantification of mRNA expression (lower). n = 9 repeats, means ± SEM, one‐way ANOVA with Tukey's multiple comparison test, ****p <.0001. (C), Representative brightfield (upper) and fluorescence (lower) images of the morphology of SH‐SY5Y cells. The quantification of the neurite mean length is shown on the right. n = 22 cells per group; data are shown as means ±SEM. All comparisons were made by one‐way ANOVA with Tukey's multiple comparison test; n.s., nonsignificant, **p <.01. (D), CM of IMRCs reduced Aβ‐induced neural cell damage in vitro. Schematic of the MTT assay (left) and the quantification of cytotoxicity by MTT assay (right). n = 5 repeats, means ±SEM, *p <.05; unpaired Student's t test