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. 2021 Jul 13;54(8):e13087. doi: 10.1111/cpr.13087

FIGURE 7.

FIGURE 7

The collagen secretion function of 3T3 cells and the changes of mTOR pathway related signalling molecules after Hst 1 treatment. A, The secretion of type I collagen in 3T3 cells after treating with Hst 1 for 3 d, vertical axis, relative gray value; horizontal axis, group. B, After treatment with Hst 1 for 3 d, the secretion of extracellular type I collagen was detected by ELISA kit, vertical axis, relative protein concentration; horizontal axis, group. C, The secretion of type III collagen in 3T3 cells after treating with Hst 1 for 3 d, vertical axis, relative gray value; horizontal axis, group. D, The possible mechanism of Hst 1 regulating fibroblast function by activating mTOR signalling pathway. E, Hst 1 was used to stimulate the cells in vitro, and the cell proteins were extracted at 15 min, 30 min, 1 h and 2 h, respectively. The expression of AKT, PI3K, mTOR and their phosphorylated proteins was detected by Western blot. Data are shown as mean ± SE. (*P < .05; **P < .01)