FIGURE 4.

Transplanted HLA‐iPSC‐NPCs contribute to the differentiation and increase myelination of corpus callosum (CC) in 11‐month‐old YAC128 transgenic mice. (A) Triple staining for the oligodendrocyte markers O4 and MBP, and hNu (Scale bar: 50 μm. White arrow: triple merged cells). (B) Immunostaining for the identification of myelination exhibited MBP‐positive area in CC (scale bar: 500 μm or 100 μm). (C) Quantification of MBP density and thickness of CC (n = 3 in each group, *P < .05). Data were analysed by two‐way ANOVA followed by a Tukey post hoc test using the SPSS software