FIG. 4.

Matrix alters cell shape and elasticity in MDA-MB-231 cell line. MDA-MB-231 cells were seeded on COL I, COL IV, FN, and LAM-coated microscope slides for 3 h. Optical tweezing was used to conduct elasticity measurements of cells corresponding to (A) round, oval, and spindle cell shapes. Each image of cells shows the polystyrene bead that was forced into the cell to measure elasticity. Elasticity results were grouped by (B) shape and (C) matrix coating. (D) Binding force assay results for COL IV, LAM, and FN. Binding force for FN was greater than the optical force generated.