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The estradiol (E2) concentration in serum before and after ovariectomy (OVX) treatment of all three types of mice. E2 concentration in wild-type (WT), estrogen receptor α knockout (ERα KO) and ERβ KO mice (A, B, C). Data were shown as average ± standard deviation (SD), n=3/groups, * indicates significant difference between group before and after OVX treatment, P<0.05, ** indicates significant difference between groups before and after OVX treatment, P<0.01, Student’s t-test, two-tailed.
