Figure 2.

Mapping genes methylated with m⁶A in prostate cancer. m⁶A was mapped in LNCaP and RWPE cell lines using miCLIP. A, Metagene plot of the distribution of m⁶A residues along the transcript in both cell lines. B, Consensus sequence surrounding m⁶A sites identified in both cell lines generated with WebLogo. C, Gene set enrichment analysis of genes ranked by the number of m⁶A sites. Shown are the top 15 gene sets. D, AR signature genes (n = 30; ref. 72) are significantly (P = 0.0004) enriched in m⁶A sites (>10 sites) as determined by Fisher exact test. E, Gene set enrichment analysis of genes ranked by the total normalized miCLIP unique tags per million for a given transcript. F, Comparing the normalized miCLIP unique tags per million per transcript in genes which are similarly expressed (<10-fold difference) between the LNCaP and RWPE cell lines.