Reproducible protein identification and quantitation from small amounts of tissue. (A,B) Venn diagrams illustrating overlap in unique protein groups (A), and unique peptides (B) identified by MaxQuant between 20, 5, and 1mg of tissue (n = 3 technical replicates for each group). (D Scatterplots of Log2 LFQ protein intensities showing high reproducibility between replicates from 20 mg (D), 5 mg (E), and 1 mg (F) tissue extractions. Pearson correlation coefficients are shown in the top left corner of each panel. (G–I) Scatterplots of Log2 LFQ protein intensities showing high reproducibility between averaged replicates from 1 mg extractions plotted against 5 mg extractions (G), 1 mg extractions plotted against 20 mg extractions (H), and 5 mg extractions plotted against 20 mg extractions (I) with Pearson correlation coefficients shown in the top left corner of each panel (n = 3 technical replicates for each group).