Principle of scGlycan-seq and scGR-seq
(A) Illustration of the DNA-barcoded lectin.
(B) Schematic representation of scGlycan-seq, scRNA-seq, and scGR-seq. Cells were incubated with DNA-barcoded lectins and separated into single cells. After DNA barcode was released by UV exposure, the released DNA barcode in the supernatants was measured by next-generation sequencing. RNA transcripts were purified from the cell pellet and analyzed by scRNA-seq.
See also Figures S1–S3.