Comparison of Glycan-seq and flow cytometry
(A) Binding of R-phycoerythrin (PE)-labeled lectins to hiPSCs (red line) and hFibs (black line) was analyzed by flow cytometry. Gray: Binding of PE-labeled BSA to hiPSCs (negative control).
(B) Binding of DNA-barcoded lectins to hiPSCs and hFibs was analyzed by Glycan-seq. The number of DNA barcode derived from lectin was divided by that of the DNA barcode of all lectins, multiplied by 100, and expressed as percentage (%). Data are shown as average ± SD of triplicates of the same sample.