Fig. 3.

Hpa2 expression is induced by MG132. (A-E) The indicated gastric carcinoma cell lines were treated with MG132 (20 µM) for the indicated time. Control cells (0) were treated with vehicle (DMSO). Total RNA was then extracted and subjected to qPCR applying primers specific for Hpa2. Hpa2 expression (fold increase) in response to MG132 treatment is shown graphically vs control untreated cells (0), set arbitrarily to a value of 1, and after normalization to actin. (F-G) Bortezomib treatment. MKN-45 cells were treated with the indicated concentration of bortezomib for 16 hours and Hpa2 expression was quantified by qPCR (F). Average Hpa2 expression (fold-increase) by bortezomib treated cells vs control (0) at a concentration of 500 nM is shown graphically (G). Hpa2, heparanase 2.