Table 3.
Cytotoxicity and photocytotoxicity of 1–7 in normoxic (~18.5% O2, top) or hypoxic (1% O2, bottom) treated SK-MEL-28 melanoma cells.
| Resazurin-based Cell Viability | ||||||||
|---|---|---|---|---|---|---|---|---|
| EC50 ± SEM (μM) | PId | |||||||
| Complex | Oxygen% | Dark | Visiblea | Greenb | Redc | Visiblea | Greenb | Redc |
| 1 | ~18.5 | 45.0 ± 7.3 | 2.81 ± 0.09 | 23.3 ± 0.8 | 31.9 ± 1.9 | 16 | 2 | 1 |
| 2 | ~18.5 | 42.3 ± 6.8 | 33.5 ± 1.1 | 34.3 ± 2.4 | 45.1 ± 7.2 | 1 | 1 | 1 |
| 3 | ~18.5 | 37.7 ± 6.3 | 27.2 ± 1.0 | 32.3 ± 3.0 | 43.2 ± 7.4 | 1 | 1 | 1 |
| 4 | ~18.5 | 36.1 ± 3.5 | 3.68 ± 0.20 | 23.1 ± 0.7 | 47.2 ± 6.0 | 10 | 2 | 1 |
| 5 | ~18.5 | 31.3 ± 1.5 | 23.5 ± 1.0 | 29.1 ± 1.2 | 34.0 ± 1.8 | 1 | 1 | 1 |
| 6 | ~18.5 | 35.9 ± 3.6 | 4.64 ± 0.20 | 21.4 ± 0.7 | 25.9 ± 0.8 | 8 | 2 | 1 |
| 7 | ~18.5 | 24.8 ± 0.9 | 2.54 ± 0.09 | 7.07 ± 0.1 | 25.8 ± 0.8 | 10 | 4 | 1 |
| 1 | 1 | 97.5 ± 6.7 | 8.66 ± 0.27 | 44.0 ± 2.7 | 56.8 ± 5.6 | 11 | 2 | 2 |
| 2 | 1 | 45.1 ± 6.8 | 44.5 ± 2.6 | 26.2 ± 1.7 | 48.5 ± 7.0 | 1 | 2 | 1 |
| 3 | 1 | 46.6 ± 5.6 | 33.6 ± 1.5 | 20.6 ± 0.6 | 49.3 ± 6.8 | 1 | 2 | 1 |
| 4 | 1 | 61.4 ± 4.7 | 21.2 ± 1.7 | 30.1 ± 1.6 | 54.8 ± 5.6 | 3 | 2 | 1 |
| 5 | 1 | 74.3 ± 2.7 | 57.6 ± 2.3 | 46.8 ± 1.1 | 65.2 ± 3.0 | 1 | 2 | 1 |
| 6 | 1 | 57.8 ± 6.7 | 32.6 ± 4.5 | 51.7 ± 4.2 | 54.8 ± 4.2 | 2 | 1 | 1 |
| 7 | 1 | 32.0 ± 0.7 | 6.95 ± 0.29 | 20.6 ± 1.0 | 28.4 ± 2.8 | 5 | 2 | 1 |
Light treatments were approximately 100 J cm−2 delivered at 18–22 mW cm−2 with acool white visible (400–700 nm), bgreen 523 nm, cred 633 nm, and dPI = phototherapeutic index. Hypoxic and normoxic experiments were ran within 4 days of each other.